Having made a whole host of changes to allow various squad members playing time, Vale lacked the batting depth to enable them to overhaul their target.
Faced with Highgate's total of 188, LMV limped to 147 as they struggled against the accuracy of the home side's bowlers on a tricky, slow wicket.
Vale would have been happy enough at the break after restricting Highgate to below 200.
David Marks (3-39) rolled back the years to mark his return to the fold, whilst Neil Lederman (3-10), bowling for the first time this season following shoulder problems, took control in the middle overs and will hope to feature more prominently as the season progresses.