Jeremy Hulsh, chief executive of Tel Aviv-based organisation Oleh! Records, helped arrange the deal for the Israeli acts to appear at the festival.
He said: “Everyone is really excited. This is a huge first and it is great for the Israeli music scene to have an influence on Glastonbury.
“The organisers have been really helpful and have given us so many stages to perform on.”
The busiest Israeli act will be soul singer Ester Rada — she is due to perform on four different stages.
As a warm-up for Glastonbury, all the groups played at the Tune In Tel Aviv concert in Islington, north London, this week.