Mr Arquette was in Israel this week filming scenes for his television series "Trippin" when he attended another barmitzvah ceremony.
The experience prompted him to agree to hold his own, and earlier today he read from the Torah and put on tefillin for the first time. The ceremony was conducted by Rabbi Shmuel Rabinovitch.
"I had my Barmitzvah today at the wall," the actor wrote on Twitter. "Finally I'm a man."
Mr Arquette has rarely commented on his Jewish background, but during his wedding in 1999 he smashed a glass in accordance with Jewish tradition.
His stay in Israel has also included visits to Masada and Tel Aviv. "This is [one] of the great cities in the world," he said of the latter.