Holders Hendon United Sports Club A will visit Maccabi London Lions Masters in the second round of this season's Peter Morrison Trophy.
David Garbacz's men have dominated the competition in recent seasons - beating league champions North London Raiders A in last season's final at Wingate & Finchley.
Raiders will host Division One high-flyers Redbridge Jewish Care B, while Redbridge's A take on Raiders C of Division Two.
There are four Maccabi London Lions teams in the competition, but this will definitely be whittled down to three in the next round as Lions A face Lions B.
Hertswood Vale, the lowest ranked team left in the competition, travel to Lions under-21's, conquerors of Scrabble FC.
PETER MORRISON TROPHY ROUND TWO (Southern)
North London Raiders A vs Redbridge Jewish Care B
Redbridge Jewish Care A vs North London Raiders C
Faithfold A vs Camden Park
Maccabi London Lions Masters vs Hendon United Sports Club A
Maccabi London Lions Under-21 vs Hertswood Vale
HMH Old Boys vs Maccabi Masters Football League
Oakwood A vs RCUK FC
SPEC FC vs FC Team A
North London Raiders B vs Woodford Wanderers
Maccabi London Lions A vs Maccabi London Lions B
Athletic Bilbaum vs Faithfold B