JNF UK has declined to say why Prime Minister David Cameron's name has disappeared from its list of honorary patrons.
Twelve days ago the pro-Palestinian Stop the JNF campaign wrote to Mr Cameron, reiterating a previous request for him to withdraw as patron of the charity.
Mortaza Sahibzada, of Stop the JNF, said: "His name has been taken off the list and that is significant. Someone has decided to take it off and I doubt whether it was JNF." Mr Cameron became an honorary patron of JNF five years ago, whose other patrons still include Tony Blair and Gordon Brown.
In its latest letter to Mr Cameron, Stop the JNF complained that the JNF British Park was planted on the ruins of two Palestinian villages after the 1948 war.
But Downing Street said that a review of all the Prime Minister's links with charities had been made when the coalition came into office. A number had been dropped, including JNF, the spokesman said, citing time constraints on Mr Cameron.